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Simple, fast and efficient key exchange
This tool allows you to exchange a key between two parties using the YubiKey Master Token. It features a no-telegraphic encryption scheme that makes the exchange safe from third-party monitoring, even in case the device is lost.
One of the most important aspects of this application is that, since it provides a centralized access to all the keys, it will not be affected by the fact that some keys were previously deleted or destroyed.
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The main purpose of this application is to provide a secure way of key exchange between several users. It relies on the YubiKey Master Token and a technique known as Merkle hash.
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This application can be a useful tool to prove ownership of a YubiKey device, since you will be able to upload a digital document containing the keys needed to recover it.
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Produce smoke, fire, firestorm, rain, or a cloud from the PhotoShop plugin Ulead Particle.Plugin.Stain-free cell counting using intensity-modulated fluorescence microscopy.
Cell counting is a routine technique in many medical and biological fields. In this paper, we present an automatic fluorescence cell counting method based on image analysis of stained cells in a microplate, using intensity-modulated fluorometry in combination with an optical system operating in the epi-mode. The microplate, containing the cells stained with a fluorochrome, is positioned in the epi-mode to have the maximum image contrast. Fluorescence intensity histograms are acquired from the acquired images of the cell plate, where these histograms are combined into a bi-dimensional representation of the stained cell area, resulting in a 2D contour plot. An automatic cell counting procedure is designed that starts by segmenting the cell contours on the 2D contour plot, and is followed by a detection of the cell centroid positions using a template matching method. For each image, three histograms are extracted from the detected cell contours: the centroid position, the centroid area, and the mean intensity. The mean intensity histogram has the lowest count-to-area ratio (100/count ratio) when compared to the cell area and the centroid position histograms, and it is used to calculate the count. This count is then compared to the known total number of cells in the microplate. A sensitivity analysis was performed to test the influence of different parameters on the performance of the algorithm. Counting errors on a standard fluorometric microplate are less than 7.5%.# Licensed to the Apache Software Foundation (ASF) under one
# or more contributor license agreements. See the NOTICE file
# distributed with this work for additional information
# regarding copyright ownership. The ASF licenses this file
# to you under the Apache License, Version 2.0 (the
# “License”); you may not use this file except in compliance
# with the License. You may obtain a copy of the License at
#
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#
#
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# Unless required by applicable law or agreed to in writing,
# software distributed under the License is distributed on an
# “AS IS” BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY

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